Biocidal compositions and use thereof

ABSTRACT

Disclosed are compositions and method for inhibiting and controlling the growth of the capsulated, facultative bacterium, Klebisella pneumoniae. The compositions comprise 2-(2-bromo-2-nitroetheny) furan and n-4-dihydroxy-oxobenzene-ethanimidoyl chloride. The method comprises adding from 1 part to about 200 parts composition to the aqueous system for which treatment is desired.

FIELD OF THE INVENTION

This invention relates to compositions and methods for controlling the growth of Klebsiella pneumoniae.

BACKGROUND OF THE INVENTION

The formation of slimes by microorganisms is a problem that is encountered in many aqueous systems. For example, the problem is not only found in natural waters such as lagoons, lakes, ponds, etc., and confined waters as in pools, but also in such industrial systems as cooling water systems, air washer systems and pulp and paper mill systems. All possess conditions which are conducive to the growth and reproduction of slime-forming microorganisms. In both once-through and recirculating cooling systems, for example, which employ large quantities of water as a cooling medium, the formation of slime by microorganisms is an extensive and constant problem.

Airborne organisms are readily entrained in the water from cooling towers and find this warm medium an ideal environment for growth and multiplication. Aerobic and heliotropic organisms flourish on the tower proper while other organisms colonize and grow in such areas as the tower sump and the piping and passages of the cooling system. The slime formation not only aids in the deterioration of the tower structure in the case of wooden towers, but also promotes corrosion when it deposits on metal surfaces. Slime carried through the cooling system plugs and fouls lines, valves, strainers, etc., and deposits on heat exchange surfaces. In the latter case, the impedance of heat transfer can greatly reduce the efficiency of the cooling system.

In pulp and paper mill systems, slime formed by microorganisms is commonly encountered and causes fouling, plugging, or corrosion of the system. The slime also becomes entrained in the paper produced to cause breakouts on the paper machines, which results in work stoppages and the loss of production time. The slime is also responsible for unsightly blemishes in the final product, which result in rejects and wasted output.

The previously discussed problems have resulted in the extensive utilization of biocides in cooling water and pulp and paper mill systems. Materials which have enjoyed widespread use in such applications include chlorine, chlorinated phenols, organs-bromines, and various organo-sulfur compounds. All of these compounds are generally useful for this purpose but each is attended by a variety of impediments. For example, chlorination is limited both by its specific toxicity for slime-forming organisms at economic levels and by the tendency of chlorine to react, which results in the expenditure of the chlorine before its full biocidal function is achieved. Other biocides are attended by odor problems and hazards with respect to storage, use or handling which limit their utility. To date, no one compound or type of compound has achieved a clearly established predominance with respect to the applications discussed. Likewise, lagoons, ponds, lakes, and even pools, either used for pleasure purposes or used for industrial purposes for the disposal and storage of industrial wastes, become, during the warm weather, besieged by slime due to microorganism growth and reproduction. In the case of industrial storage or disposal of industrial materials, the microorganisms cause additional problems which must be eliminated prior to the materials' use or disposal of the waste.

Naturally, economy is a major consideration with respect to all of these biocides. Such economic considerations attach to both the cost of the biocide and the expense of its application. The cost performance index of any biocide is derived from the basic cost of the material, its effectiveness per unit of weight, the duration of its biocidal or biostatic effect in the system treated, and the ease and frequency of its addition to the system treated. To date, none of the commercially available biocides has exhibited a prolonged biocidal effect. Instead, their effectiveness is rapidly reduced as a result of exposure to physical conditions such as temperature, association with ingredients contained by the system toward which they exhibit an affinity or substantivity, etc., with a resultant restriction or elimination of their biocidal effectiveness, or by dilution.

As a consequence, the use of such biocides involves their continuous or frequent addition to systems to be treated and their addition to multiple points or zones in the systems to be treated. Accordingly, the cost of the biocide and the labor cost of applying it are considerable. In other instances, the difficulty of access to the zone in which slime formation is experienced precludes the effective use of a biocide. For example, if in a particular system there is no access to an area at which slime formation occurs the biocide can only be applied at a point which is upstream in the flow system. However, the physical or chemical conditions, e.g., chemical reactivity, thermal degradation, etc., which exist between the point at which the biocide may be added to the system and the point at which its biocidal effect is desired render the effective use of a biocide impossible.

Similarly, in a system experiencing relatively slow flow, such as a paper mill, if a biocide is added at the beginning of the system, its biocidal effect may be completely dissipated before it has reached all of the points at which this effect is desired or required. As a consequence, the biocide must be added at multiple points, and even then a diminishing biocidal effect will be experienced between one point of addition to the system and the next point downstream at which the biocides may be added. In addition to the increased cost of utilizing and maintaining multiple feed points, gross ineconomies with respect to the cost of the biocide are experienced. Specifically, at each point of addition, an excess of the biocide is added to the system in order to compensate for that portion of the biocide which will be expended in reacting with other constituents present in the system or experience physical changes which impair its biocidal activity.

SUMMARY OF THE INVENTION

The present inventors have discovered that a composition of 2-(2-bromo-2-nitroethenyl) furan (BNEF) and n-4-dihydroxy-oxobenzene-ethanimidoyl chloride (DOEC) is effective as a biocide directed towards controlling Klebsiella pneumoniae.

DESCRIPTION OF THE RELATED ART

U.S. Pat. No. 5,158,972, Whitekettle et al., teaches the use of 2-(2-bromo-2-nitroethenyl) furan and glutaraldehyde to control the growth of Klebsiella pneumoniae. U.S. Pat. No. 4,965,371, McCoy et al., teaches a process for forming 2-(2-bromo-2-nitroethenyl furan which proved effective as an antimicrobial agent.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to compositions and methods for bacterial inhibition comprising a synergistic mixture of (A) 2-(2-bromo-2-nitroethenyl) furan and (B) n-4-dihydroxy-oxobenzene-ethanimidoyl chloride.

It has been found that mixtures of 2-(2-bromo-2-nitroethenyl) furan (BNEF) and n-4-dihydroxy-oxobenzene-ethanimidoyl chloride (DOEC) are especially efficacious in controlling the growth of bacterial microbes, specifically the Klebsiella pneumoniae species. This particular species is a member of the capsulated, facultative class of bacteria and is generally present in air, water and soil. These bacteria continually contaminate open cooling systems and pulping and papermaking systems and are among the most common slime formers. The slime may be viewed as being a mass of agglomerated cells stuck together by the cementing action of the gelatinous polysaccharide or proteinacious secretions around each cell. The slimy mass entraps other debris, restricts water flow and heat transfer, and may serve as a site for corrosion.

The fact that the Klebsiella species used in the tests is a facultative species is important, as by definition, such bacteria may thrive under either aerobic or anaerobic conditions. Accordingly, by reason of demonstrated efficacy in the growth inhibition of this particular species, one can expect similar growth inhibition attributes when other aerobic or anaerobic bacterial species are encountered. It is also expected that these compositions will exhibit similar growth inhibition attributes when fungi and algae species are encountered.

In accordance with the present invention, the combined BNEF and DOEC treatment may be added to the desired aqueous system in need of biological treatment, in an amount of from about 1 to about 200 parts of the combined treatment to one million parts (by weight) of the aqueous medium. Preferably, about 5 to about 50 parts of the combined treatment per one million parts (by weight) of the aqueous medium is added.

The combined treatment is added, for example, to cooling water systems, paper and pulp mill systems, pools, ponds, lagoons, lakes, etc., to control the formation of bacterial microorganisms, which may be contained by, or which may become entrained in, the system to be treated. It has been found that the compositions and methods of utilization of the treatment are efficacious in controlling the facultative bacterium, Klebsiella pneumoniae, which may populate these systems. The combined treatment composition and method of the present invention will also be efficacious in inhibiting and controlling all types of aerobic and anaerobic bacteria.

Surprisingly, it has been found that when the ingredients are mixed, in certain instances, the resulting mixtures possess a higher degree of bactericidal activity than that of the individual ingredients comprising the mixture. Accordingly, it is possible to produce a highly efficacious bactericide. Because of the enhanced activity of the mixture, the total quantity of the bacterial treatment may be reduced. In addition, the high degree of bactericidal effectiveness which is provided by each of the ingredients may be exploited without use of higher concentrations of each.

The invention will now be further described with reference to a number of specific examples which are to be regarded solely as being illustrative, and not as restricting the scope of the invention.

EXAMPLES

BNEF and DOEC were added in varying ratios and over a wide range of concentrations to a liquid nutrient medium which was subsequently inoculated with a standard volume of a suspension of the facultative bacterium Klebsiella pneumoniae. Growth was measured by determining the amount of radioactivity accumulated by the cells when 14C-glucose was added as the sole source of carbon in the nutrient medium. The effect of the biocide chemicals, alone and in combination, is to reduce the rate and amount of 14C incorporation into the cells during incubation, as compared to controls not treated with the chemicals. Additions of the biocides, alone and in varying combinations and concentrations, were made according to the accepted "checkerboard" technique described by M. T. Kelley and J. M. Matsen, Antimicrobial Agents and Chemotherapy. 9:440(1976). Following a two hour-incubation, the amount of radioactivity incorporated in the cells was determined by counting (14C liquid scintillation procedures) for all treated and untreated samples.

The percent reduction of each treated sample was calculated from the relationship: ##EQU1##

Plotting the % reduction of 14C level against the concentration of each biocide acting alone results in a dose-response curve, from which the biocide dose necessary to achieve any given % reduction can be interpolated.

Synergism was determined by the method of calculation described by F. C. Kull, P. C. Eisman, H. D. Sylwestrowicz and R. L. Mayer, Applied Microbiology 9,538 (1961) using the relationship: ##EQU2## wherein: Q_(a) =quantity of compound A, acting alone, producing an end point

Q_(b) =quantity of compound B, acting alone, producing an end point

Q_(A) =quantity of compound A in mixture, producing an end point

Q_(B) =quantity of compound B in mixture, producing an end point

The end point used in the calculations is the % reduction caused by each mixture of A and B. Q_(A) and Q_(B) are the individual concentrations in the A/B mixture causing a given % reduction. Q_(a) and Q_(b) are determined by interpolation from the respective dose response curves of A and B as those concentrations of A and B acting alone which produce the same % reduction as each specific mixture produced.

Dose-response curves for each active acting alone were determined by linear regression analysis of the dose-response data. Data were fitted to a curve represented by the equation shown with each data set. After linearizing the data, the contributions of each biocide component in the biocide mixtures to the inhibition of radioisotope uptake were determined by interpolation with the dose-response curve of the respective biocide. If, for example, quantities of Q_(A) plus Q_(B) are sufficient to give a 50% reduction in 14C content, Q_(a) and Q_(b) are those quantities of A or B acting alone, respectively, found to give 50% reduction in 14C content. A synergism index (SI) is calculated for each combination of A and B.

Where the SI is less than 1, synergism exists. Where the SI=1, additivity exists. Where SI is greater than 1, antagonism exists.

The data in the following tables come from treating Klebsiella pneumoniae, a common nuisance bacterial type found in industrial cooling waters and in pulping and papermaking systems, with varying ratios and concentrations of BNEF and DOEC. Shown for each combination is the % reduction of 14C content (% I), the calculated SI, and the weight ratio of BNEF and DOEC.

                  TABLE I                                                          ______________________________________                                         DOEC vs. BNEF                                                                  ppm     ppm       Ratio                                                        DOEC    BNEF      DOEC:BNEF    % I  SI                                         ______________________________________                                         20      0         100:0        89                                              15      0         100:0        84                                              10      0         100:0        76                                              5.0     0         100:0        17                                              3.75    0         100:0        11                                              2.5     0         100:0        10                                              0       160        0:100       93                                              0       100        0:100       90                                              0       80         0:100       78                                              0       50         0:100       66                                              0       20         0:100       27                                              0       10         0:100       19                                              20      160        1:8         99   1.99                                       20      100        1:5         99   1.61                                       20      80         1:4         99   1.49                                       20      50         1:2.5       99   1.31                                       20      20         1:1         97   1.17                                       20      10         2:1         94   1.15                                       15      160        1:10.7      99   1.75                                       15      100        1:6.7       99   1.37                                       15      80         1:5.3       99   1.25                                       15      50         1:3.3       99   1.06                                       15      20         1:1.3       95   0.95*                                      15      10         1.5:1       92   0.92*                                      10      160        1:16        99   1.51                                       10      100        1:10        99   1.13                                       10      80         1:8         98   1.01                                       10      50         1:5         98   0.83*                                      10      20         1:2         91   0.73*                                      10      10         1:1         85   0.75*                                      5.0     160        1:32        98   1.30                                       5.0     100        1:20        98   0.91*                                      5.0     80         1:16        97   0.79*                                      5.0     50         1:10        96   0.60*                                      5.0     20         1:4         78   0.63*                                      5.0     10         1:2         52   0.93*                                      3.75    160        1:42.7      98   1.24                                       3.75    100        1:26.7      98   0.85*                                      3.75    80         1:21.3      96   0.75*                                      3.75    50         1:13.3      95   0.56*                                      3.75    20         1:5.3       57   0.93*                                      3.75    10         1:2.7       44   0.94*                                      2.5     160        1:64        98   1.19                                       2.5     100        1:40        97   0.80*                                      2.5     80         1:32        95   0.70*                                      2.5     50         1:20        93   0.52*                                      2.5     20         1:8         50   0.93*                                      2.5     10         1:4         43   0.77*                                      ______________________________________                                    

                  TABLE II                                                         ______________________________________                                         DOEC vs. BNEF                                                                  ppm     ppm       Ratio                                                        DOEC    BNEF      DOEC:BNEF    % I  SI                                         ______________________________________                                         20      0         100:0        85                                              15      0         100:0        80                                              10      0         100:0        80                                              5.0     0         100:0        23                                              3.75    0         100:0        21                                              2.5     0         100:0        17                                              0       160        0:100       92                                              0       100        0:100       88                                              0       80         0:100       76                                              0       50         0:100       64                                              0       20         0:100       30                                              0       10         0:100       20                                              20      160        1:8         99   1.84                                       20      100        1:5         99   1.50                                       20      80         1:4         99   1.39                                       20      50         1:2.5       98   1.23                                       20      20         1:1         95   1.13                                       20      10         2:1         93   1.11                                       15      160        1:10.7      99   1.61                                       15      100        1:6.7       99   1.27                                       15      80         1:5.3       99   1.16                                       15      50         1:3.3       98   1.00                                       15      20         1:1.3       94   0.91*                                      15      10         1.5:1       91   0.90*                                      10      160        1:16        99   1.39                                       10      100        1:10        99   1.04                                       10      80         1:8         98   0.94*                                      10      50         1:5         97   0.78*                                      10      20         1:2         84   0.84*                                      10      10         1:1         81   0.80*                                      5.0     160        1:32        99   1.17                                       5.0     100        1:20        98   0.83*                                      5.0     80         1:16        97   0.73*                                      5.0     50         1:10        91   0.65*                                      5.0     20         1:4         73   0.70*                                      5.0     10         1:2         57   0.85*                                      3.75    160        1:42.7      98   1.13                                       3.75    100        1:26.7      98   0.79*                                      3.75    80         1:21.3      96   0.69*                                      3.75    50         1:13.3      90   0.60*                                      3.75    20         1:5.3       63   0.80*                                      3.75    10         1:2.7       52   0.80*                                      2.5     160        1:64        98   1.09                                       2.5     100        1:40        97   0.74*                                      2.5     80         1:32        94   0.67*                                      2.5     50         1:20        83   0.67*                                      2.5     20         1:8         58   0.76*                                      2.5     10         1:4         49   0.69*                                      ______________________________________                                    

Asterisks in the SI column indicate synergistic combinations in accordance with the Kull method supra.

In Tables I and II, differences seen between the replicates are due to normal experimental variance.

In accordance with Tables I-II supra., unexpected results occurred more frequently within the product ratios of DOEC to BNEF of from about 1.5:1 to about 1:40. Since the DOEC product contains 15% active biocidal component and the BNEF product contains 10% active biocidal component, unexpected results appear more frequently within the range of active component of (100% actives basis) DOEC:BNEF of about 2.3:1 to about 1:26.7. At present, the most preferred ratio comprises a weight ratio of active component of about 1:2 DOEC:BNEF.

While this invention has been described with respect to particular embodiments thereof, it is apparent that numerous other forms and modifications of this invention will be obvious to those skilled in the art. The appended claims and this invention generally should be construed to cover all such obvious forms and modifications which are within the true spirit and scope of the present invention. 

Having thus described the invention, what we claim is:
 1. A bacterial inhibiting composition comprising a synergistic mixture of (A) n-4-dihydroxy-oxobenzene ethanimidoyl chloride and (B) 2-(2-bromo-2-nitroethenyl) furan wherein the weight ratio of (A):(B) (100% actives basis) is from about 2.3:1 to about 1:26.7.
 2. The composition as claimed in claim 1 wherein the weight ratio of (A):(B) is about 1:2.
 3. A method for controlling the growth of Klebsiella pneumoniae bacteria in an aqueous system which comprises adding to said system from about 1 to 200 parts per weight of a composition per one million parts per weight of said aqueous system, said composition comprising a synergistic mixture of (A) n-4-dihydroxy-oxobenzene ethanimidoyl chloride and (B) 2-bromo-2-nitroethenyl) furan, the weight ratio of (A):(B) (100% actives basis) being from about 2.3:1 to about 1:26.7.
 4. The method as claimed in claim 3 wherein the weight ratio of (A):(B) is about 1:2.
 5. The method as claimed in claim 3 wherein said composition is added to said system in an amount from about 5 to about 50 parts per million of said aqueous system.
 6. The method as claimed in claim 3 wherein said aqueous system comprises a cooling water system.
 7. The method as claimed in claim 3 wherein said aqueous system comprises a pulping and papermaking system. 